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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Nutr Biochem. 2011 Aug 2;23(7):699–705. doi: 10.1016/j.jnutbio.2011.03.012

Figure 1. Decreased SIRT1 expression and increased p47phox expression in T1DM subjects compared to normal subjects.

Figure 1

Monocytes were isolated from fasting blood obtained from T1DM and normal subjects as described in Methods. For the western blot analysis, protein was subjected to SDS-PAGE and used SIRT1 and p47phox antibodies (1:1000 dilutions) as detailed in Materials and Methods Section. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for TATA binding protein (TBP) and β-actin. The immunoblot shown here are representative of 8 experiments with similar results. Densitometric ratios to TBP and Beta actin are provided in the lower panel. *p<0.01 compared to controls

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