Figure 4. Effect of resverarol on superoxide production and p47phox gene expression level in HG –induced THP-1 cells.

(A) NG, HG and HG-resveratrol treated cells were incubated with 5 µmol/L dihydroethidium (DHE). Cells (1 × 10⁵) were stained with 5 µmol/L dihydroyethidium for 30 minutes at 37°C and were washed and resuspended in PBS. The oxidative conversion of dehydroxyethidium to ethidium was measured by flow cytometry. (B) For the western blot analysis, protein was subjected to SDS-PAGE and used p47phox antibody (1:1000 dilution) as detailed in Materials and Methods Section. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for β-actin. The immunoblot shown here are representative of three independent experiments with similar results.