Detection of WGA in VTA neurons that either contain or lack TH immunoreactivity. A, B, TH-immunoreactive neurons (red cells) in the VTA and substantia nigra compacta (SNC). A′, B′, WGA-immunoreactive neurons (green cells) restricted to the VTA. Delimited areas in A′ and B′ are shown at higher magnification in panels C and C′. Transduced cells containing TH immunoreactivity are indicated by a small arrow. Large arrows indicate transfected cells lacking TH immunoreactivity. Scale bar (in C): 566 μm for A and A′; 238 μm for B and B′; 75 μm for C, C′, and merged.