TABLE 1.
Density of neurons expressing VGluT2 or TH mRNAs in the rostro-caudal sub-regions of the VTA
| Density of neurons (per mm2) expressing VGluT2 mRNA cells* |
Density of neurons (per mm2) expressing TH mRNA cells† |
|
|---|---|---|
| Rostral VTA‡ | ||
| Ventral tegmental area rostral | 338 ± 26 | 280 ± 81 |
| Parabrachial pigmental area | 62 ± 8 | 702 ± 45 |
| Medial VTA§ | ||
| Parabrachial pigmental area | 112 ± 5 | 432 ± 46 |
| Paranigral nucleus | 282 ± 34 | 183 ± 31 |
| Caudal VTA¶ | ||
| Parabrachial pigmental area | 38 ± 9 | 349 ± 25 |
| Parainterfasicular nucleus | 75 ± 16 | 651 ± 51 |
| Paranigral nucleus | 140 ± 19 | 724 ± 172 |
Data are presented as means ± SEM. Quantitative analysis at three levels of the VTA (rostral, medial and caudal) was conducted to determine the rostro-caudal density of neurons expressing VGluT2 mRNA or TH mRNA within the different subregions of the VTA (ventral tegmental area rostral, parabrachial pigmental area, parainterfasicular nucleus and paranigral nucleus). Density of cells expressing VGluT2 mRNA and density of cells expressing TH mRNA were calculated at the
rostral (ten sections, six rats),
medial (nine sections, five rats) and
caudal (four sections, three rats) levels of the VTA. Rostral VTA, −4.68 mm to −5.15 mm from Bregma; medial VTA, −5.16 mm to −5.51 mm from Bregma; caudal, −5.52 mm to −5.75 mm from Bregma.
Number of neurons expressing VGluT2 mRNA per area (density) within each VTA subregion.
Number of neurons expressing TH mRNA per area (density) within each VTA subregion.