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The 32P labeling of Complex V purified after 32P labeling in the intact mitochondria. Energized porcine heart mitochondria were incubated for 20 minutes with 32P. Mitochondrial proteins were extracted and Complex V purified using immuno-capture procedures. The insert is a zoom of the beta subunit autoradiogram of the beta subunit demonstrating the lack of correlation with the majority of the beta subunit protein spots. Experimental details in Aponte et al (Aponte et al. 2009b)
