Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
N-linked glycosylation of human CTRC. Conditioned media from transfected HEK 293T cells were analyzed by 12% SDS-PAGE and Coomassie blue staining. A. Electrophoretic mobility of wild-type CTRC and mutants N25S, N52S, and N226S. Samples were run in duplicate (left panel). Non-glycosylated CTRC produced in E. coli was used as control (right panel). B. PNGase F digestion of wild-type and mutant CTRC. C. Endo H digestion of wild-type and mutant CTRC produced in kifunensine treated cells.
