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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: FEBS J. 2011 Oct 17;278(22):4338–4350. doi: 10.1111/j.1742-4658.2011.08351.x

Figure 10.

Figure 10

Effect of engineered glycosylation sites on the glycosylation and secretion of bovine CTRC. The Asn90-Leu91-Thr92 sequon from rat CTRC and the Asn52-Asp53-Thr54 sequon from human CTRC were reconstructed in bovine CTRC by mutations E92T and D52N,N53D, respectively. A. Electrophoretic mobility of wild-type bovine CTRC and mutants E92T and D52N,N53D. Samples were run in duplicate. B. PNGase F digestion of wild-type and mutant CTRC. C. CTRC activity in the conditioned medium. Measurements were performed with the Suc-Ala-Ala-Pro-Phe-p-nitroanilide substrate, as described in Experimental Procedures. Activity was expressed as percentage of wild-type CTRC activity. Error bars indicate SEM (n=3).