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. Author manuscript; available in PMC: 2012 Oct 7.
Published in final edited form as: J Proteome Res. 2011 Sep 22;10(10):4547–4555. doi: 10.1021/pr200355w

Figure 3.

Figure 3

MyBP-C slow is phosphorylated by PKA and PKC in vitro. A: Schematic representation of the same “hypothetical” protein shown in Fig. 1A, highlighting recombinant MyBP-C slow constructs. Recombinant polypeptides are denoted as GST-NH2 terminal construct, GST-C7 construct and GST-COOH terminal construct and detected by SYPRO ruby total protein stain (B). C–F: Phosphorylation events mediated by PKA and PKC were identified in the GST-NH2-terminal construct via mass spectrometry analysis using LTQ-orbitrap. MS/MS scans of peptides carrying phosphorylated Ser-59 by PKA (C), Ser-62 by PKA (D) and Ser-204 by PKA (E) and PKC (F), identified by Bioworks-SEQUEST, with Xcorr scores of 4.471, 4.757, 4.142 and 4.475, respectively, and manually validated are shown.