Figure 3.

Modulation of inflammatory cytokine production by inhibition of autophagy with small interfering RNA (siRNA) against ATG7. Peripheral blood mononuclear cells (PBMCs) were transfected with siRNA ATG7 or scrambled siRNA after 24 hr stimulated with sonicated Mycobacterium tuberculosis (MTB) (1 μg/ml). After another 24 hr incubation tumour necrosis factor-α (TNF-α) (a) and interleukin-1β (IL-1β) (b) concentrations were measured in the supernatant using specific ELISA. Data are presented as means + SEM, n = 6.