Figure 1.

Expression of bone morphogenetic protein (BMP)/BMP antagonists in primary lymphoid organs. (a) reverse transcription-PCR analysis of BMPs, BMP antagonists in the thymus and bone marrow. Lung and kidney served as positive controls. (b) Bmp7 and Twsg1 expression on FACS sorted double-negative (DN) 2–4, double-positive (DP), single-positive (SP) CD4 and SP CD8 thymocyte populations. (c–d) FDG-based quantitative analysis of lacZ gene reporter activity by FACS on thymocyte subpopulations from Twsg1-lacZ (c), Bmp7-lacZ (d) and wild-type (wt) control mice showing significant levels of expression for Twsg1 in the SP CD4, SP CD8, DP and DN compartments in the thymus (n = 9 mice per group), while no significant differences were detected for Bmp7 (n = 5 mice per group). MFI, mean fluorescence intensity, *P < 0·05.