Identity of Twsg1 and Gremlin expressing cells in the thymus. X-gal-based detection of lacZ gene reporter activity for Twsg1 (a–d) and Gremlin (e–h) (blue) on thymus sections combined with immunohistochemical staining for for CD3 (a, e), CD25 (b, f), DEC-205 (c, g), cytokeratin8 (CK8) (d, h) (red or brown). c, cortex; m, medulla; sc, subcapsular zone (size bar 50 μm).