Figure 7.
Normal lineage distribution in Twsg1cKO mice in thymus and bone marrow. Analysis of thymocyte (a) and bone marrow (b) subpopulations and graphical representation of cell numbers from Twsg1cKO and littermate control mice. (n = 3 mice per group). The results are representative for more than three independent experiments. (a) Thymocyte populations were detected by CD4/CD8 staining and gated as double-negative (DN), double-positive (DP), CD4 single-positive (SP), CD8SP subpopulations. (b) Bone marrow populations were detected by CD31/Ly6C staining.55 Percentages and cell numbers of the various subsets were unaltered (n = 4 mice per group). The results are representative for more than three independent experiments. (c) B-cell subpopulations identified by B220/CD19/cKIT/CD25/IgM staining.2 Percentages and cell numbers of the various subsets were unaltered (n = 3 mice per group). Bl, blast-like cells; L, lymphocytes; E, erythrocytes; My, myeloid precursor cells; G, granulocytes; Mo, monocytes.