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. Author manuscript; available in PMC: 2011 Nov 7.
Published in final edited form as: Handb Exp Pharmacol. 2011;(204):251–277. doi: 10.1007/978-3-642-17969-3_11

Fig. 1.

Fig. 1

Hyperoxia increases PDE5 activity in Ovine FPASMCs. FPASMCs were exposed to 21% O2–5% CO2, 50% O2–5% CO2, or 95% O2–5% CO2 for 24 h, and total protein was harvested. PDE5-specific activity was measured as the sildenafil-inhibitable fraction of total cGMP hydrolysis, normalized for total milligrams of protein (200 µM cGMP substrate in assay; 100 nM sildenafil for inhibition). Data are shown as mean ± SEM (n = 8; read in duplicate). *P < 0.05 vs. 21% O2, #P < 0.05 vs. 50% O2. Reproduced with permission from Farrow et al. (2008a)