Table 1.

Preservation conditions and reagent composition.

Treatment	Conditions
Control	No additions; filter stored frozen dry at −80°C
RNAlater	0.5 mL of RNAlater (Ambion Inc.)
Trichloroacetic acid (TCA)	0.5 mL of 13% TCA in water
Ethanol	0.5 mL of 90% Reagent alcohol (Fisher) in water containing 1 mM EDTA.
SDS-extraction buffer	0.5 mL of reagent containing 0.1 M Tris/HCl, pH = 7.5, 1% SDS, 10 mM EDTA, 5% glycerol, incubated at 95°C for 10 min before storage.
B-PER extraction reagent	0.5 mL of B-PER reagent (Thermo-Pierce Inc.) containing 5 mM EDTA