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. Author manuscript; available in PMC: 2011 Nov 7.
Published in final edited form as: Chem Biol Drug Des. 2009 Dec 17;75(2):143–151. doi: 10.1111/j.1747-0285.2009.00921.x

Figure 2.

Figure 2

Typical set of ITC experiments performed at 25°C. The experiment on panel (A) represents the direct titration KNI-10769 (10 μL per injection of 72 μM) into the calorimetric cell (1.4272 mL) containing HIV-1 protease at a concentration of 7 μM. Panel (B) is the direct titration of KNI-10265 (10 μL per injection of 92 μM) into the cell containing HIV-1 protease at a concentration of 10 μM. The experiment on panel (C) is a displacement experiment in which KNI-10265 (10 μL per injection of 105 μM) was injected into the calorimetric cell containing HIV-1 protease pWT at a concentration of 10 μM prebound to acetyl pepstatin at a concentration of 120 μM. The experiments were performed in 10 mM sodium acetate buffer, pH 5.0, and 2% DMSO.