Diagram of the NADH-GOGAT promoter-GUS
gene constructs. A, Eight sequences containing the putative
NADH-GOGAT promoter were translational fusions to the GUS
gene; the length of these truncations is measured with respect to the
transcriptional start. The boxed region in construct G1 marks the
location of the 88-bp direct repeat. The number of individual
transformants tested for each construct is indicated (#). Mean GUS
activity (pmol min−1 mg−1 protein) is
indicated with the heading “Mean.” lsd values between
adjacent deletions were calculated, and the level of significance was
set at P < 0.01. N.S., Not significant; *, significant at P
< 0.01. B, Distribution of GUS activity per construct measured from
individual independently transformed plants.