Figure 5.

Relative tags from ChIPSeq indicate three states of RNAPII. (a) The average Ser5P RNAPII enrichment tags per one gene from ChIPseq data, normalized to input by Peakseq, were summed according to their shifted positions with the definition that gene length was from 0 to 1. Where 0 represents the transcription start site and 1 represents the transcription end site. This indicated three states of RNAPII: Low pausing and low elongation, high pausing and low elongation, and high pausing and high elongation. (b) Ser2P was also analyzed. No significant enrichment was seen, except for Ser2P and Ser5P double positive genes.