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. Author manuscript; available in PMC: 2012 Oct 27.
Published in final edited form as: J Med Chem. 2011 Sep 22;54(20):7150–7164. doi: 10.1021/jm200739e

Figure 5. FRα and β binding assays for assorted antifolates.

Figure 5

Data are shown for the effects of the unlabeled ligands with FRα-expressing RT16 CHO cells and FRβ-expressing D4 CHO cells. Relative binding affinities for assorted folate/antifolate substrates were calculated as the inverse molar ratios of unlabeled ligands required to inhibit [3H]folic acid binding by 50%. By definition, the relative affinity of folic acid (FA) is 1. Results are presented as mean values plus/minus SEM from 3 experiments. Experimental details are provided in the Experimental Section.