Venn diagram showing distribution of transcripts before and after heat exposure. (A) shows control C57BL/6 and AKR/N testes; (B) shows control C57BL/6 testis and 2 heat-resistant stains: AKR/N and MRL/MpJ-+/+. Circle a: number of transcripts regulated within 24 hours by heat, including transcripts that exhibited significant changes in expression levels as defined by our parameters between any time points (8, 10, 12 and 24 hours) and control, in AKR/N mouse testis. Circle b: number of transcripts regulated within 24 hours by heat, including transcripts that exhibited significant changes between any time points (8, 10, 12, and 24 hours) and control in C57BL/6 mouse testis. Circle c: number of transcripts differentially expressed between control C57BL/6 and AKR/N testes. Circle d: number of transcripts differentially expressed between control C57BL/6 testis and 2 heat-resistant stains, AKR/N and MRL/MpJ-+/+.