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. 2011 Aug 26;30(19):4071–4083. doi: 10.1038/emboj.2011.293

Figure 2.

Figure 2

Fear extinction regulates hippocampal Igf2/Igfbp7 expression. (A) Mice were subjected to fear extinction. Behavioural analysis confirmed that freezing behaviour was significantly reduced upon ELF exposure, when compared to E1. Mice that were subjected to the conditioning context but not subjected to fear extinction served as control (*P<0.05 versus E1; n=15/group). (B) qPCR analysis of Igf2 and Igfbp7 expression in the total hippocampus at different time points after fear extinction training (*P<0.05 versus control; n=5/group). (C) qPCR analysis of Igf2 and Igfbp7 expression in the dentate gyrus (*P<0.05 versus control; n=5/group). (D) Representative images showing immunostaining for IGF2 and IGFBP7 protein levels in the dentate gyrus. Scale bars low magnification images: 50 μm; high magnification: 2 μm. (E) Left panel: Representative immunoblot analysis of IGF2 and IGFBP7 protein levels in the CA region 3 h after exposure to E1 or ELF. Right panel. Quantification (n=5/group). (F) Left panel: Representative immunoblot analysis of IGF2 and IGFBP7 protein levels in the dentate gyrus region 3 h after exposure to E1 or ELF. Right panel. Quantification (*P<0.05 versus control, n=5/group). DGlb, dentate gyrus lateral blade; DGmb, dentate gyrus medial blade; E, extinction trial; ELF, extinction trial low freezing; gc, granular cell layer of the dentate gyrus; Po, polymorph layer of the dentate gyrus; error bars indicate s.e.m.