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. 2011 Aug 23;30(19):3977–3993. doi: 10.1038/emboj.2011.308

Figure 7.

Figure 7

Vreteno is a novel Yb-body component. (A) Subcellular localization of GFP-tagged Vreteno or Armitage (optical section of egg chambers) expressed under the respective endogenous regulatory regions. (B) Confocal section through the follicular epithelium of a GFP–Vreteno (green) expressing egg chamber stained for Armitage (red) and DNA (blue). (Right panel) Merge of all three channels (co-localization of Vreteno and Armitage results in yellow). (C) Immunostaining of Vreteno (green), Piwi (red) and DNA (blue) in egg chambers, where clones of cells mutant for the indicated genes (left) have been induced in the follicular epithelium (clone borders are indicated with a yellow line). (D) Co-immunostaining of Vreteno (left) and Armitage (right) in OSCs transfected with siRNAs against EGFP (top), Yb (middle) or zucchini (bottom). (E) Co-IPs of Armitage and Vreteno from OSC lysate. Western blots against Armitage, Vreteno, Piwi and Yb. For the Armitage western blot (Armi-IP), only 1/15th compared with all other blots was loaded in the IP-lanes. Beads lacking antibody (IP-beads) served as control.