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. 2011 Sep 13;30(19):3962–3976. doi: 10.1038/emboj.2011.328

Figure 2.

Figure 2

FoxA1 depletion in VCaP cells. (A) FoxA1 mRNA and protein levels. VCaP cells were treated for 72 h with siRNA specific for FoxA1 mRNA (siFoxA1) or control siRNA (parental). (B) Overlap of ARBs (FDR <2%) in parental and FoxA1-depleted VCaP cells. (C) Directed ChIP validation of new ARBs in parental (white bars) and FoxA1-depleted (solid bars) VCaP cells. The cells were exposed to 100 nM DHT (+) or vehicle (−) for 2 h prior to ChIP assays. Mean+s.e.m. values for duplicate samples are shown.