Table 1.
Data collection and refinement statistics
| Native | Ni2+ peak | Ni2+ remote | |
|---|---|---|---|
| Data collection | |||
| X-ray Source | ALS 8.3.1 | ALS 8.3.1 | ALS 8.3.1 |
| Wavelength | 1.1159 | 1.4855 | 1.4370 |
| Space group | P 41212 | P 41212 | P 41212 |
| Cell dimensions | |||
| a, b, c (Å) | 113.39, 113.39, 88.22 | 112.75, 112.75, 86.85 | |
| α, β, γ (°) | 90.00, 90.00, 90.00 | 90.00, 90.00, 90.00 | |
| Resolution (Å) | 50-3.55(3.64-3.55)* | 50-4.70 (4.82-4.70) | 50-4.70 (4.82-4.70) |
| Rsym | 6.8 (75.7) | 5.9 (91.3) | 5.4 (77.9) |
| I / σ | 17.46 (3.02) | 23.81 (3.44) | 26.38 (4.01) |
| Completeness (%) | 99.5 (99.8) | 99.8 (100.0) | 99.8 (100.0) |
| Redundancy | 11.2 (8.7) | 15.2 (15.5) | 15.1 (15.4) |
| Refinement | |||
| Resolution (Å) | 3.55 | ||
| No. reflections | 82099 | ||
| Rwork / Rfree | 0.221/0.254 | ||
| No. atoms | |||
| RNA | 1796 | ||
| Ligand/ion | 17 | ||
| B-factors | |||
| RNA | 148.5 | ||
| Ligand/ion | 165.8 | ||
| r.m.s. deviations | |||
| Bond lengths (Å) | 0.008 | ||
| Bond angles (°) | 0.769 |
*
Values in parentheses are for highest-resolution shell.