Table 2.
Translation activities of IRES mutants
| Mutant | Translation Activity a | Mutant | Translation Activity a |
|---|---|---|---|
| WT | 100 ± 7 %b | WT | 100 ± 7 %b |
| SII penult 5′x | 19 ± 4 % | A136G | 134 ± 16 % |
| SII penult 3′x | 74 ± 11 % | A136C | 134 ± 6 % |
| SII penult comp | 61 ± 5 % | A136U | 99 ± 10 % |
| SII ult 5′x | 103 ± 4 % | A288U | 20 ± 3 % |
| SII ult 3′x | 75 ± 7 % | U297A | 104 ± 8 % |
| SII ult comp | 70 ± 8 % | A288U,U297A | 32 ± 9 % |
| SII end comp | 50 ± 1 %c | A288C | 98 ± 10 % |
| SI end GU | 98 ± 12 % | U297G | 24 ± 3 % |
| SI end AU | 93 ± 7 % | A288C,U297G | 50 ± 4 % |
| SI penult 5′x | 38 ± 4 % | A288G | 107 ± 5 % |
| SI penult 3′x | 87 ± 3 % | U297C | 118 ± 12 % |
| SI penult comp | 109 ± 4 % | A288G,U297C | 93 ± 8 % |
| SI ult 5′x | 106 ± 4 % | SII top 3′x | 40 ± 9 %c |
| SI ult 3′x | 84 ± 4 % | x A288G | 22 ± 4 % |
| SI ult comp | 100 ± 4 % | x U297C | 10 ± 3 % |
| x A288G, U297C | 45 ± 12 % | ||
| x A136G | 50 ± 3 % | ||
| x A136C | 32 ± 1 % |
a
reported as the mean and standard deviation between four translation reactions from two independent transcriptions.
b
WT error is the mean deviation of WT from all duplicate translation reactions.
c
previously reported (Berry et al., 2010)