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. 2011 Aug 25;20(23):4515–4529. doi: 10.1093/hmg/ddr381

Figure 1.

Figure 1.

Four-kiloDalton Aβ is secreted into the media of AβPP-derived neurons. Twenty-five micrograms of the cell lysate or 10 µl of conditioned cell media from WT and AβPP cultures (16 DIV) was resolved on a 10–20% tricine gel, and AβPP and Aβ proteins were detected using AβPP- and Aβ-specific antibody (6E10, which recognizes Aβ residues 1–16) after following standard immunoblotting procedure. Left panel, media from the AβPP and WT cultures show an accumulation of 4 kDa Aβ monomer and 100 kDa-secreted AβPP. Right panel, cell lysates from AβPP and WT cultures show an accumulation of 110 kDa full-length AβPP and 16 kDa Aβ oligomer.