Figure 2.

Aβ oligomer accumulates in cell neurites and soma over time. Double-immunocytochemistry with the A11 (anti-oligomer: green) and 6E10 (red) antibodies revealed 6E10 immunoreactivity present at all time points examined (10, 16, 22 DIV), indicated by increased staining in the cell body by 22 DIV. A11 immunoreactivity was not seen in appreciable amounts at 10 DIV, but was found extensively throughout neurites at 16 DIV. By 22 DIV, A11 staining was found within the cell body. WT neurons did not exhibit specific staining with the 6E10 or A11 antibodies. Images were all collected with a 100× objective.