Agarose gel Electrophoresis pattern of isolated Lactobacillus DNA. Two microliter DNA samples were run in each lane of a 0.9% Agarose gel. a Genomic DNA isolated by the present protocol and by a commercial kit using a similar volume of isolates grown in MRS broth (C1, C2 and C3). Lanes 1–3 Lactobacillus DNA isolated by the commercial kit; Lanes 5–7, DNA isolated by the present protocol; Lane M, HindIII digested Lambda DNA fragments (23.13, 9.4, 6.5, 4.3, 2.3, 2.0, 0.5 and 0.12 kb) b Agarose gel electrophoretic profile of Lactobacillus genomic DNA isolated using the present protocol