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Line scan of native PAGE gels for SOD activity in leaf extracts of transgenic alfalfa plants expressing pMitSOD or pChlSOD. Cu/Zn-SOD1 and Cu/Zn-SOD2 designate cytosolic and chloroplastic forms of SOD, respectively. Fe-SOD activity was observed as a small band only on some gels. The area of the Mn-SOD band was increased in both pMitSOD and pChlSOD compared with N4. A distinct double band was observed in Mn-SOD of pChlSOD but not pMitSOD.
