Table V.

Electrolyte leakage from leaves and roots after freezing of independent transgenic alfalfa plants expressing pMitSOD or pChlSOD

Main Treatment Effects	Electrolyte Leakage
	Leaf	Root
	% of total
Acclimation (weeks)
2	55	74
4	42	70
4 + 1a	NAb	56
lsd (0.05)	0.2 (n = 108)	0.2 (n = 108)
Temperature (°C)
−6	33	58
−7	49	67
−8	53	70
−9	58	73
lsd (0.05)	0.3 (n = 54)	0.3 (n = 81)
Genotype
Control N4  pMitSOD	51	68
N4 pMitSOD-1	53	67
N4 pMitSOD-6	52	66
N4 pMitSOD-9	48	66
N4 pMitSOD-10	47	66
pChlSOD
N4 pChlSOD-1	47	64
N4 pChlSOD-6	44	66
N4 pChlSOD-7	46	70
N4 pChlSOD-9	45	68
lsd (0.05)	0.6 (n = 24)	0.5 (n = 36)

The experiment was factorial with a split plot design of three acclimation stages (main plot-different weeks) × 7 plants × 4 temperatures × 3 replications (different days). Leaf and root samples were analyzed separately. Values for the main treatment effects are averaged across other treatments in the factorial experiment.

^aPlants were acclimated at 2°C for 4 weeks, frozen at −2°C, and then acclimated at 2°C for 1 more week. Leaves were injured by the −2°C acclimation treatment. 

^bNA, Data not available.