Table 2.
Enzyme assay | 0 h (Control) | 24 h | 36 h | 48 h |
---|---|---|---|---|
Lignin peroxidasea (Intracellular) | 0.245 ± 0.034 | 0.289 ± 0.004** | 0.367 ± 0.040** | 0.274 ± 0.025 |
Lignin peroxidasea (Extracellular) | 0.034 ± 0.010 | 0.045 ± 0.002* | 0.067 ± 0.003*** | 0.083 ± 0.040*** |
Laccaseb (Intracellular) | 0.012 ± 0.01 | 0.016 ± 0.001** | 0.023 ± 0.004*** | 0.019 ± 0.02** |
NADH–DCIP reductasec | 14.30 ± 0.59 | 15.46 ± 0.61 | 32.49 ± 0.63*** | 18.19 ± 0.63* |
MG reductased | 18.00 ± 0.81 | 17.62 ± 0.74 | 16.81 ± 0.72 | 14.02 ± 0.31 |
Riboflavin reductasee | 0.89 ± 0.07 | 2.33 ± 0.05** | 5.23 ± 0.11*** | 6.14 ± 0.11*** |
aμmoles of formaldehyde produced/mg of protein/min
bUnits of enzyme/mg of protein/min
cμg of DCIP reduced/mg of protein/min
dμg of MG reduced/mg of protein/min
eμg of riboflavin reduced/mg of protein/min
Significantly different than the control at * P < 0.05, ** P < 0.01, *** P < 0.001 by Tukey–Kramer Multiple Comparison Test. Data was analyzed by one-way ANOVA, values are mean of three experiments, SEM (±)