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. 2011 Jan 25;51(4):501–508. doi: 10.1007/s12088-011-0131-4

Table 2.

Enzyme activities at different time intervals during decolorization process of amaranth

Enzyme assay 0 h (Control) 24 h 36 h 48 h
Lignin peroxidasea (Intracellular) 0.245 ± 0.034 0.289 ± 0.004** 0.367 ± 0.040** 0.274 ± 0.025
Lignin peroxidasea (Extracellular) 0.034 ± 0.010 0.045 ± 0.002* 0.067 ± 0.003*** 0.083 ± 0.040***
Laccaseb (Intracellular) 0.012 ± 0.01 0.016 ± 0.001** 0.023 ± 0.004*** 0.019 ± 0.02**
NADH–DCIP reductasec 14.30 ± 0.59 15.46 ± 0.61 32.49 ± 0.63*** 18.19 ± 0.63*
MG reductased 18.00 ± 0.81 17.62 ± 0.74 16.81 ± 0.72 14.02 ± 0.31
Riboflavin reductasee 0.89 ± 0.07 2.33 ± 0.05** 5.23 ± 0.11*** 6.14 ± 0.11***

aμmoles of formaldehyde produced/mg of protein/min

bUnits of enzyme/mg of protein/min

cμg of DCIP reduced/mg of protein/min

dμg of MG reduced/mg of protein/min

eμg of riboflavin reduced/mg of protein/min

Significantly different than the control at * P < 0.05, ** P < 0.01, *** P < 0.001 by Tukey–Kramer Multiple Comparison Test. Data was analyzed by one-way ANOVA, values are mean of three experiments, SEM (±)