Reaction 2b (lanes1–5) and Reaction 3 (lanes 8–12 (Table 5A). Lanes 1 and 8 QUBC43 Shigella (API), lanes 2 and 9 QUBC42 Salmonella (API), lanes 3 and 10 QUBC41 Klebsiella (API), lanes 4 and 11 QUBC40 Proteus, and lanes 5 and 12 QUBC34 Staphylococcus aureus. Notice the presence of the 510 bp in lanes3–5 and its absence from lanes 1 and 2 and the presence of a 630 bp band in lanes 8 and 9 and its absence in lanes 10, 11, and 12