Table 6.
PCR product size with paired primers based on published rDNA gene H. pylori (HP) HPAG1 gi|108562424:c1140006-1138506 or P. fluorescens (PF) gi|70728250:122811-124349 of Pf-5 NC_004129
| Forward primer | QUGP-F1 | QUGP-Fn3 | QUGP-F4 | QUGP-Fn5 | QUGP-Fn6 |
|---|---|---|---|---|---|
| Reverse primer | HP/PF | HP/PF | HP/PF | HP/PF | HP/PF |
| QUGP-Rn1 | 1463/1503 | 721/743 | 633/639 | 1142/1183 | 995/991 |
| 1277b | |||||
| QUGP-Rn2 | 00/1396 | 623/636 | 532/536 | 00/1073 | 00/893 |
| 1097b | |||||
| QUGP-Rn3 | 764/798 | NPa | NP | 465/473 | 287/298 |
| QUGP-R4 | 847/881 | 112 | NP | 530/561 | 374/379 |
| QUGP-Rn5 | 334/344 | NP | NP | NP | NP |
| QUGP-Rn6 | 488/523 | NP | NP | 171/199 | NP |
The exact PCR product size for each primer pair may vary from one bacterium to another. Some primer sites are missing and their products are indicated as zero (00 bp)
aPrimers that did not form PCR pairs are indicated as no pairing (NP). The exact PCR product size depends on bacterial species. Italicized products indicate size similarity
bHomo sapiens 18S sequence shares homologies with these primers and predicts the possible amplification of 1277 and 1097 bp region