Validation of autoantibody biomarkers using commercially available ELISA kits. (A) The fluorescence signals of four autoantibodies, anti-SSA, anti-SSB, anti-transglutaminase, and anti-histone, measured by the protein microarrays. The mean fluorescence values from duplicates of the array spots are reported in RFU, and mean group value is given by the horizontal bar. (B) ELISA of anti-SSA, anti-SSB, anti-transglutaminase, and anti-histone, in pSS patients (n=34), SLE patients (n=34), and healthy control subjects (n=34).