Table I.
Primary Antibodies
| Antigen | Immunogen | Manufacturer | Dilution |
|---|---|---|---|
| β2/3 | affinity-purified bovine GABAAR | Mouse monoclonal hybridoma supernatant, clone 62-3G1, made in our laboratory | 1:5 for EM postembedding |
| GAD | Purified rat GAD | Sheep polyclonal, gift from Dr. Irwin J. Kopin, Lot# 1440-4 | 1:500 for immunofluorescence |
| γ2 | AA 1–15 of rat GABAAR γ2 subunit | Guinea pig polyclonal, made in our laboratory | 1:1000 for immunofluorescence |
| gephyrin | Affinity-purified rat glycine receptor protein complex | Synaptic Systems, Cat# 147011, clone mAb7a, Lot# 147011/14, mouse monoclonal | 1:500 for immunofluorescence |
| GFAP | Bovine spinal cord homogenate | PharMingen, Cat# 60311D, clone 4A11, Lot# MO50728, mouse monoclonal | 1:600 for immunofluorescence |
| GLAST | Synthetic peptide from the carboxy-terminus of rat GLAST (QLIAQD NEPEKPVADSETKM) | Millipore, Cat# AB1782, Lot# 22061280, Guinea pig polyclonal | 1:2000 for immunofluorescence |
| GluR2 | Recombinant fusion protein TrpE-GluR2 (N-terminal portion, AA 175–430) | Millipore, Cat# MAB397, mouse monoclonal, clone 6C4, Lot# 19090047 | 1:100 for EM postembedding |
| MAP2 | Recombinant MAP2 protein | Novus, Cat# NB300-213, Lot# D1, Chicken polyclonal | 1:20000 for immunofluorescence |
| Pan-Pcdh -γ | AA 808–931 (C-terminal constant domain) of mouse Pcdh-γA1 | N e u r o M a b, C a t # 73–185, mouse monoclonal, clone N159/5, Lot# 437-3VA-24 | 1:15 for immunofluorescence |
| Pan-Pcdh -γA | AA 720–804 (variable cytoplasmic domain) of mouse Pcdh-γA3 | NeuroMab, Cat#73-178, mouse monoclonal, clone N144/32, Lot# 437-3VA-12 | 1:15 for immunofluorescence |
| Pcdh-γC5 | AA 1–14 (N-terminus variable domain) of rat Pcdh- γC5 | Rabbit polyclonal, made in our laboratory | 1:50 to 1:30 for immunofluorescence; 1:100 for light microscopy immunocytochemistry; 1:3 to 1:5 for EM postembedding; 1:75 to 1:150 for EM preembedding and 1:5 for immunoblot |
| PSD-95 | GST fusion protein encoding the full-length rat PSD-95 | Chicken polyclonal antiserum UCT-C1, gift from Dr. Randall S. Walikonis | 1:1000 for immunofluorescence |
| PSD-95 | Recombinant rat PSD-95 | Millipore, Cat# MAB 1596, Lot# LV1453199, mouse monoclonal, clone 6G6-1C9 | 1:100 for immunofluorescence |
| vGAT | Synthetic peptide (SLEGLIEAYRTNAED) of rat vGAT | Chemicon, Cat# AB5855, Lot# 24030142, Guinea pig polyclonal | 1:2000 for immunofluorescence |
| vGlut1 | Synthetic peptide (GATHSTVQPPRPPPPVRDY) of rat vGLUT1 | Chemicon, Cat# AB5905; Lot# 24080852, Guinea pig polyclonal | 1:10000 for immunofluorescence |
| S-100β | Purified bovine brain S-100β preparation | Sigma, Cat# S2532, Lot# 085K4880, clone SH-B1, mouse monoclonal | 1:2000 for immunofluorescence |