Figure 1.

a, Partial restriction map of the 3429-bp cDNA encoding barley limit dextrinase. b, Overlapping cDNAs and PCR products used to obtain the complete nucleotide sequence of the cDNA for barley limit dextrinase. The 2.6-kb 3′ cDNA was screened from a library of GA₃-treated aleurone layers. The 316-bp “central” sequence was obtained as a PCR product amplified from cDNA inserts excised from the same aleurone cDNA library. The 507-bp 5′ fragment was prepared by 5′ RACE PCR from aleurone total RNA. The arrows at the 3′ end indicate multiple polyadenylation sites.