Figure 4.

Promoter region of the barley limit dextrinase gene. a, Nucleotide sequence of the gene upstream from the translation start ATG codon. The putative transcription start point is indicated by the arrowhead and is designated nucleotide +1. Underlined sequences starting from the 5′ end of the sequence include the CCTTT pyrimidine box, the TAAAACAAA box and the TATCCAA box of the putative GA₃ response element, and the CATT box and the proposed TATA box of the gene. b, Primer extension from total RNA of GA₃-treated aleurone layers using a primer specific for the 5′ region of the cDNA. The top two lanes show the major primer extension (PE) termination products (arrows), whereas the lower four lanes represent the nucleotide sequence of the gene, primed with the same oligonucleotide used for the primer extension.