Figure 5.

Northern blots of RNA preparations from developing barley grain and GA₃-treated aleurone layers using limit dextrinase cDNA as a probe. a, Lanes 1 to 4, RNA from grain during the period 19 to 40 DPA (grain weight 27–70 mg); lanes 5 and 6, RNA from aleurone layers treated for 48 h with (+GA) or without (−GA) 2 μm GA₃. b, Time course of GA₃ induction of limit dextrinase mRNA transcripts in isolated barley aleurone layers.