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IEF of extracts of a barley developing grain (A) and a germinated grain (B). The left panels show proteins stained with Coomassie brilliant blue and the right panels show limit dextrinase activity revealed by clearing of a Red Pullulan overlay gel. Lanes S, pI protein standards; lanes M, mature grain and other extracts from developing endosperm 8, 10, and 16 DPA. The number of days after the initiation of germination are indicated in B.
