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. 2011 Oct 10;8:79. doi: 10.1186/1742-4690-8-79

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Copyright ©2011 Huang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Autoprocessing of precursors with different N-terminal tags. The GST portion in the GST-p6*-PR-HA construct (Figure 2A) was replaced with a GCN motif (left), GFP (middle), or Hsp70 (right), respectively. The resulting precursors also contained a Flag peptide upstream of the D site. Blots were probed using either mouse anti-Flag (upper) or mouse anti-HA (lower) primary antibodies. The dotted lines connect the products expected from D site cleavage reaction and solid lines connect the products from P site cleavage reaction. The GCN4- containing products were too small to detect by SDS-PAGE.