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. 2011 Nov 7;6(11):e27083. doi: 10.1371/journal.pone.0027083

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Aachoui, Ghosh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mice were immunized with phthalate-KLH conjugate emulsified in different adjuvants. Serum samples were collected as described under Materials and Methods and diluted to 1∶1000 in 0.5% PBS/BSA. Anti-phthalate, anti-KLH, and anti-DNA antibody levels were determined using ELISA as described. The results represent the mean ± SD (n = 6 mice per group in two separate experiments). The significance in experimental groups was determined relative to the group given antigen only (no adjuvant group) at the level of p≤0.05.