Figure 4. Cdc34 S97 phosphorylation increases in the G1 phase of the cell cycle.

Strain RC150 was grown to mid-log phase and arrested with a final concentration of 0.5 µg/ml µM of α-factor. After 3 hours, >95% of the cells had arrested with a shmoo-like morphology. Cells were washed and resuspended in fresh YPD. Cells were collected at the indicated time points and total soluble protein was analyzed by western blot using the α-pS97 phospho-specific and α-Cdc34 antibody. At least 100 cells were counted per time point to determine the budding index.