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. 2011 Nov 7;6(11):e27148. doi: 10.1371/journal.pone.0027148

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Araujo-Palomares et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Fluorescent images show the localization of YFP-CDC-42, YFP-RAC and GFP-CDC-24 during spore germination and germ tube emergence. White arrows indicate the presence of YFP-CDC-42 at discrete region of the spore indicated a new polarization site and indicate that CDC-42 continues present at apical dome during germ tube emergence. Black arrow indicates the localization of YFP-RAC during germ tube elongation. White arrowhead indicates the localization of GFP-CDC-24 at discrete region of the spore and germ tube elongation. (B) Phase-contrast and Fluorescent images showing the localization of YFP-CDC-42, YFP-RAC and GFP-CDC-24 in germlings of N. crassa. Note that CDC-42 and RAC are present in the septum development in germlings, but CDC-24 was not detected in this process. White arrowheads show septum formation by phase-contrast and fluorescent microscopy. Black arrowhead indicates the localization of YFP-CDC-42, YFP-RAC and GFP-CDC-24 at the apex of germlings.