Figure 2. Sensorgrams of wild-type and R689C MSPβ binding to immobilized RON extracellular domain constructs.
The binding curves in each sensogram correspond to increasing concentrations of the analyte in 2-fold dilution steps: 4, 8, 16, 32, 64, 128, 256, and 512 nM. The eight experiments correspond to immobilized: (A,E) RON Sema. (B,F) RON Sema-PSI. (C,G) RON Sema-PSI-IPT1. (D,H) RON Sema-PSI-IPT1–4. The response units (RU) were corrected for nonspecific binding relative to a blank flow cell surface.
