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. 2011 Nov 7;6(11):e27318. doi: 10.1371/journal.pone.0027318

Figure 1. Inhibition of tumor growth by 16K hPRL is associated with an increased vessel number but with decreased vessel perfusion.

Figure 1

(A) The data represent mean tumor volumes ± SEM in 16K-Ad-treated (n = 8) or Null-Ad-treated (n = 10) mice. (B) The number of tumor vessels per field ± SEM was determined on CD31-positive vessels in x100 power fields of each section. Bar, 100 µm. (C) Representative photographs of tumor sections from 16K-Ad- and Null-Ad-inoculated mice, double-stained for the endothelial cell marker CD31 (red) and the perfusion marker lectin (green). White arrows, vessels stained positive for CD31 and lectin. Heads of white arrows, vessels only positive for CD31. (D) The perfusion index was calculated as the percentage of lectin-positive vessels (perfused vessels) per CD31-positive vessels in the B16F10 tumors ± SEM. For (B) and (D) the data represent, for each set of conditions, the mean ratio ± SEM calculated for all the fields of all tumor sections (one section per tumor). *, P<0.05.