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. 2011 Nov 7;6(11):e27318. doi: 10.1371/journal.pone.0027318

Figure 6. Decreased expression of Notch factors in HUVEC cocultured with HBVP after 16K hPRL treatment.

Figure 6

(A–E) HUVEC pretreated for 30 min with 100 nM 16K hPRL (16K) were cultured for 6 h in the presence or absence of HBVP. The two cell populations were then separated prior to qRT-PCR analysis and subjected to qRT-PCR analysis to detect DLL4, NOTCH4, NOTCH1, HEY2 and EPHRINB2 transcripts. (F) Western blot analysis to detect Notch1 ICD. Data are presented as means ± SEM, n = 3 and are representative of at least two different experiments. n.s., non significant. *, P<0.05; ***, P<0.0001.