Figure 5. Drosophila Ku70 mutation accelerates locomotion disability of mutant Htt transgenic flies.

Startle-induced negative geotactic response (time to reach to 5 cm high) was investigated. Control, heterozygous DmKu70 mutants, transheterozygous mutants, and their Htt103Q expressing flies were tested at 3, 7 and 14 days after eclosion. Htt103Q expressers and non-expressers were compared separately. Significant difference was observed between the HD background and its compound heterozygous mutant both in male and female flies. The numbers of individual fly used for the analysis (N) are indicated above each bar. Experiments were performed three times with each fly, thus the actual “ n ” for statistical analysis was three times of the fly number (N). We employed ANOVA and posthoc Dunnett's test to compare them. *: p<0.05.