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. 2011 Nov 7;6(11):e27424. doi: 10.1371/journal.pone.0027424

Figure 4. PP2A activity is increased in hepatocytes treated with palmitate.

Figure 4

□ Control; ▪ 0.5 mM Palmitate. The protein level of a PP2A structural subunit (PP2Aα) was upregulated in hepatocytes treated with palmitate (a). Meanwhile, PP2A activity was increased by approximately 30 per cent in insulin-resistant palmitate-treated hepatocytes (b, c). PP2A-activity was estimated by the degree of methylation of the PP2A catalytic subunit (b) or a direct phosphatase activity assay (c). Protein levels were normalized to β–actin. Data are averages of western blot quantifications or PP2A activity measurements +/− std. error of the mean, n = 10. * indicates p<0.05. Representative western blots are shown for a and b.