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. 2011 Nov 7;6(11):e27425. doi: 10.1371/journal.pone.0027425

Figure 1. Expression of selected CIS genes in MMTV-induced mammary tumors.

Figure 1

RNA was extracted from eight MMTV-induced mammary tumors; 5 from C3H mice (BF, BN, C, F, P) and 2 from BALB/c mice (5, 11). Normal mammary gland (MG) was obtained from a 4 month old virgin mouse and used as a control. Expression of selected CIS genes (Wnt1, Prkaca, Arhgap18, Tcf7l2, Antxr1) were measured by qRT-PCR. GAPDH was used as the endogenous control gene. The relative levels of the gene specific PCR product were normalized to GAPDH. All error bars represent standard deviations. Arrows indicate tumors harboring the corresponding CIS.