Figure 2. Expression of target CIS proteins in transduced mammary epithelial cells and enhanced cell growth.

The pBabe retroviral constructs containing the cDNAs of Wnt1, Arhgap18, Tcf7l2, and Antxr1 were transduced into NMuMG and HC11 mouse mammary cells. A) Cell extracts from the different transduced cells were subjected to western blot analysis using anti-HA antibodies. B) Cells stably transduced with CIS genes were plated in 96-well plates and grown for 4 days. Cell growth was monitored by the MTT assay (Materials and Methods). Data represents mean values with the SE (n = 5). Data were analyzed by two way ANOVA analysis with Dunnett's post hoc test. At day 4, all CIS-transduced NMuMG cells and all but the Tcf7l2-transduced HC11 cells showed significant differences from control cells (p≤0.01).