Figure 4. Increased expression of CIS genes causes morphological transformation of mammary epithelial cells in 3D culture.

A) NMuMG or HC11 cells transduced with the different CIS genes or empty vector (control) were grown in 8 well chamber slides with matrigel. Images were captured at day 10 (200X magnification). B) Acinar size was measured on day 10 of growth in matrigel. Acini with diameters over 50 µm were scored (n = 30 for each cell line). **p≤0.01, determined by Student's t-test.